Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with F-Testo(Free Testosterone) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to F-Testo(Free Testosterone). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-Testo(Free Testosterone) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：F-TESTO; Free Testosterone
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：0.48 pg/mL
Standard：100 pg/mL
Detection range：1.57-100 pg/mL
Sample type：serum, plasma, other biological fluids
