Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAE(14-3-3 protein epsilon). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to YWHAE(14-3-3 protein epsilon). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YWHAE(14-3-3 protein epsilon), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of YWHAE(14-3-3 protein epsilon) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：14-3-3 E; 14-3-3 epsilon; FLJ45465; MDCR; YWHAE; protein kinase C inhibitor protein 1; MDS; KCIP 1
Species：Human
Assay Type：Sandwich
Sensitivity：26.5 pg/mL
Standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：serum, plasma and other biological fluids
