Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide(CAMP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide(CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide(CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathelicidin Antimicrobial Peptide(CAMP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
Species：Cattle
Assay Type：Sandwich
Sensitivity：0.27 ng/mL
Standard：50 ng/mL
Detection range：0.79-50 ng/mL
Sample type：serum, plasma and other biological fluids
