Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apurinic/Apyrimidinic Endonuclease 1(APEX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apurinic/Apyrimidinic Endonuclease 1(APEX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apurinic/Apyrimidinic Endonuclease 1(APEX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apurinic/Apyrimidinic Endonuclease 1(APEX1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：APE; APE1; APEN; APEX; APX; HAP1; REF1; Multifunctional DNA Repair Enzyme; APEX Nuclease 1; Redox factor-1; DNA-(apurinic or apyrimidinic site) lyase
Species：Rat
Assay Type：Sandwich
Sensitivity：0.058 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
