Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIIIP(procollagen Ⅲ propeptide). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIIIP(procollagen Ⅲ propeptide). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIIIP(procollagen Ⅲ propeptide), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIIIP(procollagen Ⅲ propeptide) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：procollagen 3 propeptide; P3P; _x005f procollagen III propeptide; PIIIP
Species：Rat
Assay Type：Sandwich
Sensitivity：11.5 pg/mL
Standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：Serum, plasma and other biological fluids
