Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with p-hydroxyphenyllactic acid (HPLA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to p-hydroxyphenyllactic acid (HPLA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p-hydroxyphenyllactic acid (HPLA) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：HPLA
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：6.65ng/mL
Standard：1000 ng/mL
Detection range：15.6-1000 ng/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
