Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 4 Alpha(MIP4a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 4 Alpha(MIP4a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 4 Alpha(MIP4a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 4 Alpha(MIP4a) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CCL26; SCYA26; IMAC; TSC-1; Eotaxin 3; Thymic stroma chemokine-1; Chemokine(C-C-Motif)ligand 26; Thymic Stroma Chemokine-1; Small Inducible Cytokine Subfamily A 26
Species：Mouse
Assay Type：Sandwich
Sensitivity：6.1 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
