Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cardiolipin(CL) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cardiolipin(CL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cardiolipin(CL) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：1,3-Bis(sn-3-Phosphatidyl)-Sn-Glycerol
Species：Dog
Assay Type：Competitive Inhibition
Sensitivity：1.5 ng/mL
Standard：1000 ng/mL
Detection range：15.63-1000 ng/mL
Sample type：tissue homogenates and other biological fluids
