Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Humanin-Like Protein 6 (MTRNR2L6) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Humanin-Like Protein 6 (MTRNR2L6). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Humanin-Like Protein 6 (MTRNR2L6) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:MTRNR2L6; Humanin-like 6; HN6; MT-RNR2-like protein 6
Species:Human
Assay Type:Competitive Inhibition
Sensitivity:0.151 ng/mL
Standard:20 ng/mL
Detection range:0.32-20 ng/mL
Sample type:serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids