Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I N-Terminal Propeptide(PINP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide(PINP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I N-Terminal Propeptide(PINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide(PINP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
Species：Bovine
Assay Type：Sandwich
Sensitivity：1.19 ng/mL
Standard：200 ng/mL
Detection range：3.13-200 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
