Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B- and T-lymphocyte attenuator(BTLA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B- and T-lymphocyte attenuator(BTLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B- and T-lymphocyte attenuator(BTLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B- and T-lymphocyte attenuator(BTLA) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names： B and T lymphocyte associated protein; B and T lymphocyte attenuator; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA_HUMAN; BTLA1; CD272; CD272 antigen; FLJ16065; MGC129743
Species：Human
Assay Type：Sandwich
Sensitivity：0.22 ng/mL
Standard：30 ng/mL
Detection range：0.47-30 ng/mL
Sample type：Serum, plasma, urine, tissue homogenates, cell culture supernates and other biological fluids
