Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXCL1(Chemokine C-X-C Motif Ligand 1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CXCL1(Chemokine C-X-C Motif Ligand 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CXCL1(Chemokine C-X-C Motif Ligand 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CXCL1(Chemokine C-X-C Motif Ligand 1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：NAP3; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein
Species：Human
Assay Type：Sandwich
Sensitivity：5.9 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：serum, plasma and other biological fluids
