Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CTXI(Cross-linked Carboxy-terminal telopeptide of type I collagen). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CTXI(Cross-linked Carboxy-terminal telopeptide of type I collagen). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CTXI(Cross-linked Carboxy-terminal telopeptide of type I collagen), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CTXI(Cross-linked Carboxy-terminal telopeptide of type I collagen) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names： Cross-linked Carboxy-terminal telopeptide of type 1 collagen; CTX-1; Cross-linked Carboxy-terminal telopeptide of type I collagen; CTX-I; Cross linked C terminal Telopeptides of type I collagen
Species：Human
Assay Type：Sandwich
Sensitivity：0.266 ng/mL
Standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：serum, plasma and other biological fluids
