Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with NEFA(Non-Esterified Fatty Acid) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NEFA(Non-Esterified Fatty Acid). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEFA(Non-Esterified Fatty Acid) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Non-Esterified Fatty Acid; Non-ester fatty acid; NEFA
Species：Bovine
Assay Type：Competitive Inhibition
Sensitivity：3.55 nmol/ml
Standard：400nmol/ml
Detection range：6.25-400 nmol/ml
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
