Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CA 27-29(cancer antigen 27-29). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CA 27-29(cancer antigen 27-29). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CA 27-29(cancer antigen 27-29), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CA 27-29(cancer antigen 27-29) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CA 27-29
Species：Human
Assay Type：Sandwich
Sensitivity：0.8 U/mL
Standard：100 U/mL
Detection range：1.57-100 U/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
