Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Androsterone(ADT) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Androsterone(ADT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Androsterone(ADT) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：17-Ketosteroid; 17-KS; 3a-Hydroxy-17-Androstanone
Species：Pig
Assay Type：Competitive Inhibition
Sensitivity：144 pg/mL
Standard：90000 pg/mL
Detection range：1406.25-90000 pg/mL
Sample type：serum, plasma and other biological fluids
