Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MTOR(Serine/threonine-protein kinase mTOR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MTOR(Serine/threonine-protein kinase mTOR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MTOR(Serine/threonine-protein kinase mTOR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MTOR(Serine/threonine-protein kinase mTOR) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target
Species：Pig
Assay Type：Sandwich
Sensitivity：0.125 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
