Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SP-D(Pulmonary surfactant-associated protein D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SP-D(Pulmonary surfactant-associated protein D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SP-D(Pulmonary surfactant-associated protein D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SP-D(Pulmonary surfactant-associated protein D) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SFTPD; SPD; SFTP 4;
Species：Human
Assay Type：Sandwich
Sensitivity：0.52 ng/mL
Standard：80 ng/mL
Detection range：1.25-80 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids
