Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Delta(FIL1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Delta(FIL1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Delta(FIL1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Delta(FIL1d) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：IL1HY1; IL36RN; IL36Ra; IL1RP3; IL1L1; IL1F5; Interleukin 1 Family, Member 5; Interleukin-1 HY1; IL-1 Related Protein 3; Interleukin 36 Receptor Antagonist
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.57 pg/mL
Standard：100 pg/mL
Detection range：1.57-100 pg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
