Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-Hydroxydeoxyguanosine(8-OHdG) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 8-Hydroxydeoxyguanosine(8-OHdG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-Hydroxydeoxyguanosine(8-OHdG) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：26.29 pg/mL
Standard：6000 pg/mL
Detection range：93.75-6000 pg/mL
Sample type：serum, plasma and other biological fluids
