Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Obestatin(OB) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Obestatin(OB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Obestatin(OB) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Obestatin
Species：Pig
Assay Type：Competitive Inhibition
Sensitivity：25.1 pg/mL
Standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
