Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Alpha(IL8Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 Receptor Alpha(IL8Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Alpha(IL8Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Alpha(IL8Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CD181; CDw128a; CXCR1; CKR1; CMKAR1; IL8R1; IL8RBA; IL8-RA; Chemokine C-X-C motif Receptor 1; High Affinity Interleukin-8 Receptor A
Species：Pig
Assay Type：Sandwich
Sensitivity：0.054 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates and other biological fluids
