Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PI3K(Phosphotylinosital 3 kinase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PI3K(Phosphotylinosital 3 kinase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PI3K(Phosphotylinosital 3 kinase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PI3K(Phosphotylinosital 3 kinase) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：PI3K; Phosphotylinosital 3 kinase;
Species：Rat
Assay Type：Sandwich
Sensitivity：3.9 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Tissue homogenates and other biological fluids.
