Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histidyl-tRNA synthetase, cytoplasmic(HARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histidyl-tRNA synthetase, cytoplasmic(HARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histidyl-tRNA synthetase, cytoplasmic(HARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histidyl-tRNA synthetase, cytoplasmic(HARS) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：FLJ20491, HRS, HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
Species：Human
Assay Type：Sandwich
Sensitivity：5.4 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates and other biological fluids
