Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pro-Interleukin 1 Beta(pro-IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to pro-Interleukin 1 Beta(pro-IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pro-Interleukin 1 Beta(pro-IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pro-Interleukin 1 Beta(pro-IL1b) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：pro-IL1-B;pro-IL1-Beta; pro-IL1F2; pro-IL-1β
Species：Rat
Assay Type：Sandwich
Sensitivity：16.4 pg/mL
Standard：4000 pg/mL
Detection range：62.5-4000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates and other biological fluids
