Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p-cresol. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：4-Hydroxytoluene; 4-Methylphenol; p-Cresol 98+ %; PARA-CRESOL; 4-cresol; para cresol
Species：Human
Assay Type：Sandwich
Sensitivity：1.21 pg/mL
Standard：200 pg/mL
Detection range：3.13-200 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
