Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 6-keto-PGF1 alpha protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 6-keto-PGF1 alpha. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 6-keto-PGF1 alpha in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：6-keto-PGF1 alpha; 6ketoPGF1α
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：1.4 pg/ml
Standard：500 pg/ml
Detection range：7.8 pg/ml - 500 pg/ml
Sample type：Serum, plasma and other biological fluids.
