Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tyrosol(Tyr) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosol(Tyr). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosol(Tyr) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:Tyrosol
Species:General
Assay Type:Competitive Inhibition
Sensitivity:142.7 ng/mL
Standard:30000 ng/mL
Detection range:468.75-30000 ng/mL
Sample type:biological agents and other biological fluids