Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin(HB) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hemoglobin(HB). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Hgb; Haemoglobin; Heterotetramer(αβ)2
Species：Cattle
Assay Type：Competitive Inhibition
Sensitivity：5.57 μg/mL
Standard：1000 μg/mL
Detection range：15.63-1000 μg/mL
Sample type：Serum, plasma, erythrocyte lysates and other biological fluids
