Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-Hydroxyeicosatetraenoic Acid(12-HETE) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 12-Hydroxyeicosatetraenoic Acid(12-HETE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-Hydroxyeicosatetraenoic Acid(12-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：12HETE
Species：General
Assay Type：Competitive Inhibition
Sensitivity：5.32 ng/mL
Standard：1000 ng/mL
Detection range：15.63-1000 ng/mL
Sample type：serum, plasma and other biological fluids
