Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylserine(PS) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylserine(PS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylserine(PS) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:Ptd-L-Ser; PtdSer
Species:General
Assay Type:Competitive Inhibition
Sensitivity:5.27 ng/mL
Standard:1200 ng/mL
Detection range:18.75-1200 ng/mL
Sample type:Serum, plasma and other biological fluids