Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with S-Adenosyl Methionine(SAM) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S-Adenosyl Methionine(SAM). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S-Adenosyl Methionine(SAM) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SAMe; SAM-e; S-Adenosylmethionine; S-(5'-Adenosyl)-L-Methionine Chloride
Species：General
Assay Type：Competitive Inhibition
Sensitivity：4.84 ng/mL
Standard：1000 ng/mL
Detection range：15.63-1000 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
