Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 1(RSPO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 1(RSPO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 1(RSPO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 1(RSPO1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：RSPONDIN; Roof plate-specific spondin-1
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.054 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：tissue homogenates, cell lysates, cell culture supernates and other biological fluids
