Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type II(IL1R2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type II(IL1R2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type II(IL1R2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Type II(IL1R2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CD121b; CD121-B; IL1-R2; IL1RB; Interleukin-1 Receptor Beta; CD121 Antigen-like Family Member B
Species：Simian
Assay Type：Sandwich
Sensitivity：0.213 ng/mL
Standard：40 ng/mL
Detection range：0.63-40 ng/mL
Sample type：Serum, plasma and other biological fluids.
