Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Cytosolic II(NT5C2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5'-Nucleotidase, Cytosolic II(NT5C2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Cytosolic II(NT5C2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Cytosolic II(NT5C2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：NT5B; PNT5; GMP; cN-II; NT5CP; Purine 5' Nucleotidase; 5'-Nucleotidase(Purine),Cytosolic Type B; Cytosolic purine 5'-nucleotidase
Species：Rat
Assay Type：Sandwich
Sensitivity：0.128 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
