Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesoid X Receptor(FXR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Farnesoid X Receptor(FXR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Farnesoid X Receptor(FXR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Farnesoid X Receptor(FXR) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：NR1H4; BAR; FXR; HRR1; RIP14; Nuclear Receptor Subfamily 1,Group H,Member 4; Bile acid receptor; Farnesol receptor HRR-1; Retinoid X receptor-interacting protein 14
Species：Rat
Assay Type：Sandwich
Sensitivity：0.053 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
