Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with V-Set And Immunoglobulin Domain Containing Protein 2(VSIG2) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Set And Immunoglobulin Domain Containing Protein 2(VSIG2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Set And Immunoglobulin Domain Containing Protein 2(VSIG2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:CTXL; CTH; Cortical thymocyte-like protein
Species:Human
Assay Type:Competitive Inhibition
Sensitivity:152.5 pg/mL
Standard:40000 pg/mL
Detection range:625-40000 pg/mL
Sample type:Tissue homogenates and other biological fluids.