Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Initiator 1(REPIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Replication Initiator 1(REPIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Initiator 1(REPIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Initiator 1(REPIN1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：AP4; RIP60; ZNF464; Zfp464; Replication Initiation Region Protein(60kD); Zinc Finger Protein 464; 60 kDa origin-specific DNA-binding protein; ATT-binding protein
Species：Mouse
Assay Type：Sandwich
Sensitivity：0.135 ng/mL
Standard：20 ng/mL
Detection range：0.32-20 ng/mL
Sample type：Tissue homogenates and other biological fluids.
