Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ghrelin-O-Acyltransferase(GOAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ghrelin-O-Acyltransferase(GOAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ghrelin-O-Acyltransferase(GOAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ghrelin-O-Acyltransferase(GOAT) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：OACT4; MBOAT4; Membrane Bound O-Acyltransferase Domain Containing Protein 4
Species：Human
Assay Type：Sandwich
Sensitivity：0.31 ng/mL
Standard：50 ng/mL
Detection range：0.79-50 ng/mL
Sample type：serum, plasma, tissue homogenates and other biological fluids
