Test principle:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Parathymosin(PTMS) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parathymosin(PTMS). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parathymosin(PTMS) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:ParaT
Species:Mouse
Assay Type:Competitive Inhibition
Sensitivity:14.2 ng/mL
Standard:3000 ng/mL
Detection range:46.88-3000 ng/mL
Sample type:Serum, plasma and other biological fluids.