Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Secretagogin(SCGN) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretagogin(SCGN). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretagogin(SCGN) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：SECRET; SEGN; CALBL; Calbindin-Like; Secretagogin,EF-Hand Calcium Binding Protein
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：24.7 pg/mL
Standard：5000 pg/mL
Detection range：78.13-5000 pg/mL
Sample type：Serum, plasma and other biological fluids.
