Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serum Amyloid P Component(SAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serum Amyloid P Component(SAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serum Amyloid P Component(SAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serum Amyloid P Component(SAP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：APCS; PTX2; Pentaxin-Related; 9.5S Alpha-1-Glycoprotein
Species：Rat
Assay Type：Sandwich
Sensitivity：2.44 ng/mL
Standard：400 ng/mL
Detection range：6.25-400 ng/mL
Sample type：Serum, plasma and other biological fluids.
