86-7877-73　Pig NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit 96T　ELK6163

特徴

• Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP) in the samples is then determined by comparing the OD of the samples to the standard curve.

仕様

• サイズ：96T
• Alternative Names：NT-Pro-BNP; ; N-BNP
• Species：Pig
• Assay Type：Sandwich
• Sensitivity：0.063 ng/mL
• Standard：10 ng/mL
• Detection range：0.16-10 ng/mL
• Sample type：Serum, plasma and other biological fluids
• Assay length：4.5h
• Research Area：
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