Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neuropeptide FF(NPFF) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropeptide FF(NPFF). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide FF(NPFF) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：FMRFAL; Neuropeptide FF-Amide Peptide Precursor; Pro-FMRFamide-related neuropeptide FF
Species：Mouse
Assay Type：Competitive Inhibition
Sensitivity：13.5 pg/mL
Standard：500 pg/mL
Detection range：7.82-500 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
