Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G2(IgG2) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Immunoglobulin G2(IgG2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G2(IgG2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：IGHG2; Ig Gamma-2 Chain C Region; Immunoglobulin Heavy Constant Gamma 2; G2m Marker; Immunoglobulin Gm2
Species：Guinea pig
Assay Type：Competitive Inhibition
Sensitivity：124.5 ng/mL
Standard：25000 ng/mL
Detection range：390.63-25000 ng/mL
Sample type：Serum, plasma and other biological fluids.
