Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 2',5'-Oligoadenylate Synthetase 1(OAS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 2',5'-Oligoadenylate Synthetase 1(OAS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 2',5'-Oligoadenylate Synthetase 1(OAS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 2',5'-Oligoadenylate Synthetase 1(OAS1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：2,5OAS1; 25-OAS1; OAS1; IFI-4; OIAS; OIASI; E18/E16
Species：Rat
Assay Type：Sandwich
Sensitivity：0.32 ng/mL
Standard：50 ng/mL
Detection range：0.79-50 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
