Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha(IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha(IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha(IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Alpha(IL1a) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：IL1 Alpha，IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
Species：Horse
Assay Type：Sandwich
Sensitivity：6.2 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
