Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Alpha(IL2Ra). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 2 Receptor Alpha(IL2Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Alpha(IL2Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 2 Receptor Alpha(IL2Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen
Species：Rat
Assay Type：Sandwich
Sensitivity：0.059 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：serum, plasma, tissue homogenates, cell lysates and other biological fluids
