86-7871-41　［取扱停止］Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit 96T　ELK5535

特徴

• Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Neurofilament, Light Polypeptide(NEFL) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament, Light Polypeptide(NEFL). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament, Light Polypeptide(NEFL) in the samples is then determined by comparing the OD of the samples to the standard curve.

仕様

• サイズ：96T
• Alternative Names：CMT1F; CMT2E; NF-L; NF68; NFL; 68 kDa neurofilament protein; Neurofilament triplet L protein
• Species：Human
• Assay Type：Competitive Inhibition
• Sensitivity：46.4 pg/mL
• Standard：10000 pg/mL
• Detection range：156.25-10000 pg/mL
• Sample type：serum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids
• Assay length：2h
• Research Area：
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