Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-Hemoglobin Stabilizing Protein(aHSP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-Hemoglobin Stabilizing Protein(aHSP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-Hemoglobin Stabilizing Protein(aHSP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-Hemoglobin Stabilizing Protein(aHSP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：A-HSP; ERAF; EDRF; Erythroid Associated Factor; Erythroid differentiation-related factor
Species：Mouse
Assay Type：Sandwich
Sensitivity：13.8 pg/mL
Standard：2000 pg/mL
Detection range：31.25-2000 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
